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genome wide ko eko sgrna library  (Addgene inc)


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    Addgene inc genome wide ko eko sgrna library
    <t>Genome-wide</t> <t>CRISPR-Cas9</t> screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.
    Genome Wide Ko Eko Sgrna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome+wide+ko+eko+sgrna+library/pUT1854-HQ858793+(Plasmid+%2327875)/pmc12860261-49-27-21
    Average 94 stars, based on 5 article reviews
    genome wide ko eko sgrna library - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract"

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract

    Journal: Redox Biology

    doi: 10.1016/j.redox.2026.104047

    Genome-wide CRISPR-Cas9 screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.
    Figure Legend Snippet: Genome-wide CRISPR-Cas9 screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.

    Techniques Used: Genome Wide, CRISPR, Knock-Out, Labeling

    EGCG-induced pro-oxidant mechanisms: Insights from genome-wide CRISPR screening. (1) EGCG undergoes auto-oxidation generating reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and quinone intermediates. A genome-wide CRISPR-Cas9 screen identified key genes modulating cellular response to EGCG-induced oxidative stress. (2) Knockouts of glutathione biosynthesis genes ( GCLC , GCLM , GSS ) impair H 2 O 2 detoxification, sensitizing cells to ferroptosis. (3) Peroxisomal genes ( PEX1 , PEX6 , PEX12 , PEX14 ) regulate ROS metabolism; their disruption compromises H 2 O 2 clearance via catalase ( CAT ) and peroxiredoxin-1 ( PRDX1 ). (4) The KEAP1-NRF2 axis controls antioxidant gene expression, inducing enzymes (e.g., CAT, PRDX1, GCLs) that mitigate ROS toxicity. KEAP1 knockout enhances NRF2 signaling and confers resistance. (5) Additional modulators include ABCC1 (drug efflux), SLC7A11 (cysteine transporter) and BAK1 (apoptosis). Sensitizer hits (red labels) indicate knockouts that heighten EGCG toxicity, while resistance hits (green labels) protect against cell death. Color intensity reflects CRANKS scores relative to the highest scoring genes. Together, these findings illustrate how EGCG's pro-oxidant activity can overwhelm cancer cell defenses when redox-regulating pathways are genetically compromised.
    Figure Legend Snippet: EGCG-induced pro-oxidant mechanisms: Insights from genome-wide CRISPR screening. (1) EGCG undergoes auto-oxidation generating reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and quinone intermediates. A genome-wide CRISPR-Cas9 screen identified key genes modulating cellular response to EGCG-induced oxidative stress. (2) Knockouts of glutathione biosynthesis genes ( GCLC , GCLM , GSS ) impair H 2 O 2 detoxification, sensitizing cells to ferroptosis. (3) Peroxisomal genes ( PEX1 , PEX6 , PEX12 , PEX14 ) regulate ROS metabolism; their disruption compromises H 2 O 2 clearance via catalase ( CAT ) and peroxiredoxin-1 ( PRDX1 ). (4) The KEAP1-NRF2 axis controls antioxidant gene expression, inducing enzymes (e.g., CAT, PRDX1, GCLs) that mitigate ROS toxicity. KEAP1 knockout enhances NRF2 signaling and confers resistance. (5) Additional modulators include ABCC1 (drug efflux), SLC7A11 (cysteine transporter) and BAK1 (apoptosis). Sensitizer hits (red labels) indicate knockouts that heighten EGCG toxicity, while resistance hits (green labels) protect against cell death. Color intensity reflects CRANKS scores relative to the highest scoring genes. Together, these findings illustrate how EGCG's pro-oxidant activity can overwhelm cancer cell defenses when redox-regulating pathways are genetically compromised.

    Techniques Used: Genome Wide, CRISPR, Disruption, Gene Expression, Knock-Out, Activity Assay

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    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
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    Generated:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) . ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract.
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Transduction:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) . ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract.
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

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    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Genome Wide:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) . ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract.
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..



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    genome wide ko eko sgrna library  (Addgene inc)
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    Addgene inc genome wide ko eko sgrna library
    <t>Genome-wide</t> <t>CRISPR-Cas9</t> screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.
    Genome Wide Ko Eko Sgrna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome+wide+ko+eko+sgrna+library/pUT1854-HQ858793+(Plasmid+%2327875)/pmc12860261-49-27-21
    Average 94 stars, based on 1 article reviews
    genome wide ko eko sgrna library - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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    Genome-wide CRISPR-Cas9 screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.

    Journal: Redox Biology

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract

    doi: 10.1016/j.redox.2026.104047

    Figure Lengend Snippet: Genome-wide CRISPR-Cas9 screen identifies genetic determinants of GTE-induced cytotoxicity. (A) Schematic overview of the experimental workflow for the genome-wide CRISPR knockout screen in NALM-6 cells treated with green tea extract (GTE), aimed at identifying genes modulating compound sensitivity. (B) Distribution of CRANKS scores for 19,034 genes following GTE treatment. Negative scores (red arrows, CRANKS ≤ −2) indicate sensitizer genes whose knockout enhances compound sensitivity, while positive scores (green arrows, CRANKS ≥2) represent rescuers whose knockout confers resistance. The 2D scatter plot is aligned with a one-dimensional frequency distribution, illustrating the thresholds used to define top hits. (C) Volcano plot showing CRANKS scores plotted against –log 10 p -values. Genes above the horizontal dashed line marks the p -threshold ( p < 0.05), while those beyond the vertical dashed lines exceed CRANKS thresholds of ±2, identifying 30 filtered genes ( p ≤ 0.01) labeled by gene symbol. Red points represent sensitizers; green points represent rescuers. Listed starred gene (∗) indicate top hits. (D) KEGG pathway enrichment analysis of highest CRANKS-filtered candidates (|CRANKS| ≥ 2; p ≤ 0.01; set including all top hits). Bubble color reflects false discovery rate (FDR), ranging from light green (FDR = 9 × 10 −7 ) to dark blue (FDR = 4 × 10 −3 ), and bubble size corresponds to the number of genes in each pathway. Pathways are ranked by significance on the y-axis. Analysis was performed using the STRING database with whole-genome background, requiring a minimum of two genes per pathway and FDR ≤0.05.

    Article Snippet: Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs).

    Techniques: Genome Wide, CRISPR, Knock-Out, Labeling

    EGCG-induced pro-oxidant mechanisms: Insights from genome-wide CRISPR screening. (1) EGCG undergoes auto-oxidation generating reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and quinone intermediates. A genome-wide CRISPR-Cas9 screen identified key genes modulating cellular response to EGCG-induced oxidative stress. (2) Knockouts of glutathione biosynthesis genes ( GCLC , GCLM , GSS ) impair H 2 O 2 detoxification, sensitizing cells to ferroptosis. (3) Peroxisomal genes ( PEX1 , PEX6 , PEX12 , PEX14 ) regulate ROS metabolism; their disruption compromises H 2 O 2 clearance via catalase ( CAT ) and peroxiredoxin-1 ( PRDX1 ). (4) The KEAP1-NRF2 axis controls antioxidant gene expression, inducing enzymes (e.g., CAT, PRDX1, GCLs) that mitigate ROS toxicity. KEAP1 knockout enhances NRF2 signaling and confers resistance. (5) Additional modulators include ABCC1 (drug efflux), SLC7A11 (cysteine transporter) and BAK1 (apoptosis). Sensitizer hits (red labels) indicate knockouts that heighten EGCG toxicity, while resistance hits (green labels) protect against cell death. Color intensity reflects CRANKS scores relative to the highest scoring genes. Together, these findings illustrate how EGCG's pro-oxidant activity can overwhelm cancer cell defenses when redox-regulating pathways are genetically compromised.

    Journal: Redox Biology

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract

    doi: 10.1016/j.redox.2026.104047

    Figure Lengend Snippet: EGCG-induced pro-oxidant mechanisms: Insights from genome-wide CRISPR screening. (1) EGCG undergoes auto-oxidation generating reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and quinone intermediates. A genome-wide CRISPR-Cas9 screen identified key genes modulating cellular response to EGCG-induced oxidative stress. (2) Knockouts of glutathione biosynthesis genes ( GCLC , GCLM , GSS ) impair H 2 O 2 detoxification, sensitizing cells to ferroptosis. (3) Peroxisomal genes ( PEX1 , PEX6 , PEX12 , PEX14 ) regulate ROS metabolism; their disruption compromises H 2 O 2 clearance via catalase ( CAT ) and peroxiredoxin-1 ( PRDX1 ). (4) The KEAP1-NRF2 axis controls antioxidant gene expression, inducing enzymes (e.g., CAT, PRDX1, GCLs) that mitigate ROS toxicity. KEAP1 knockout enhances NRF2 signaling and confers resistance. (5) Additional modulators include ABCC1 (drug efflux), SLC7A11 (cysteine transporter) and BAK1 (apoptosis). Sensitizer hits (red labels) indicate knockouts that heighten EGCG toxicity, while resistance hits (green labels) protect against cell death. Color intensity reflects CRANKS scores relative to the highest scoring genes. Together, these findings illustrate how EGCG's pro-oxidant activity can overwhelm cancer cell defenses when redox-regulating pathways are genetically compromised.

    Article Snippet: Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs).

    Techniques: Genome Wide, CRISPR, Disruption, Gene Expression, Knock-Out, Activity Assay